Asian Journal of Bio Science

Vikas Sharma ¹, Madhulika Bhagat ²

Affiliations
1 Division of Biochemistry and Plant Physiology, Sher-E Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, Jammu (J&K), IN
2 School of Biotechnology, University of Jammu, Jammu (J&K), IN

Abstract

Cancer still remains the major cause of mortality and morbidity all over the world. In the search of potential anticancer agents from the medicinal plants, the present research work was carried out to examine the anticancer properties of Azadirachta indica leaves and stem bark against human cancer cell lines via., ethanolic, hydro-ethanolic and aqueous extracts using sulphorhodamine B (SRB) dye. All the three extracts from leaf part and stem-bark showed in vitro cytotoxicity against all the human cancer cell lines at 100 μg/ml. At lower doses (10 and 30 μg/ml) aqueous extract from leaf part was found to be more active than ethanolic and hydro-ethanolic extracts in dose dependent manner. Results showed the potent anticancer effect of Azadirachta indica (leaves and stem bark) on human cancer cell lines of colon, liver, neurablastoma and prostate origin and the plant can be explored for probable anticancer lead molecules for the drug development.

Keywords

Azadirachta indica, Human Cancer Cell Lines, In vitro Cytotoxicity, Anticancer.

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Madhulika Bhagat ¹, Jatinder Singh Arora ², Ajit Kumar Saxena ³

Affiliations
1 School of Biotechnology, University of Jammu, Jammu (J & K), IN
2 Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar (Punjab), IN
3 Pharmacology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi (J & K), IN

Abstract

Calotropis procera (Ait.) R.Br., a popular medicinal species in Indian system of medicine, is widely used for the treatment of various diseases. In the present investigation the cytotoxicity of the three extracts and their fractions from aerial parts of Calotropis procera was evaluated against central nervous system (SNB-78) human cancer cell line. The results showed that hydro-alcohloic extract has more potential at three different concentrations of 10, 30 and 100 μg/ml, among all the three extracts viz., alcoholic, hydro-alcoholic and aqueous extracts. On evaluation of the various fractions, n-butanol fraction of both hydro-alcoholic and alcoholic extracts had shown antiproliferative activity against human cancer cell line of central nervous system (SNB-78) at three different concentrations of 10 and 30 μg/ml. Aqueous extract was found to be least active.

Keywords

Calotropis Procera, Aerial Parts, Extracts, Fractions, Cytotoxicity, Human Cancer Cell Line.

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Madhulika Bhagat ¹, Jatinder Singh Arora ², Ajit Kumar Saxena ³

Affiliations
1 Department of Biotechnology, University of Jammu, Jammu (J&K), IN
2 Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar (Punjab), IN
3 Pharmacology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu (J&K), IN

Abstract

Ficus hispida Linn. is explored for its in vitro cytotoxicty against oral (KB) and colon (COLO 205) human cancer cell lines. Three extracts (alcoholic, hydro-alcoholic and aqueous) and four fractions (n-hexane, chloroform, n-butanol and aqueous) from the leaves were prepared and were evaluated using sulforhodamine B assay at 10, 30 and 100 μg/ml. The growth inhibition demonstrated by all extracts and fractions were in dose dependent manner. The alcoholic extract was most active followed by aqueous and 50% aqueous-alcoholic extract. Further on the evaluation of fractions, n-butanol fraction was highly significant among the four fractions of alcoholic extract against both oral and colon cancer cell lines.

Keywords

Ficus hispida, Cytotoxicity, SRB Assay, KB Human Cancer Cell Lines.

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Vikas Sharma ¹, Madhulika Bhagat ²

Affiliations
1 Division of Biochemistry and Plant Physiology, Sher-e Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, Jammu (J&K), IN
2 School of Biotechnology, University of Jammu, Jammu (J&K), IN

Abstract

River water was assayed in the city of Allahabad. Amongst the parameters used were BOD, DO, pH, conductivity and heavy metals, viz., Cu, Cd, Cr, Fe, Zn, Pb, Ni, Mn. Site specific water samples were collected from the ghats of Ganga, Yamuna, Sangam and subjected to analysis. Physico-chemical characteristics of the water samples indicated a lower level of pollution in the water of Yamuna river whereas the confluence (Sangam) was found to be most polluted.

Keywords

Physico-Chemical, Heavy Metals, Water Samples, Pollution.

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